Fwd: High-resolution melting analysis for rapid screening of BRCA1 and BRCA2 Spanish mutations.

---------- Forwarded message ----------
From: HubMed - breast cancer <rssfwd@rssfwd.com>
Date: Sat, Jun 7, 2008 at 2:25 AM
Subject: High-resolution melting analysis for rapid screening of BRCA1 and BRCA2 Spanish mutations.
To: mesothelioma77@gmail.com


[1]Breast Cancer Res Treat. 2008 Jun 5;
de Juan I, Esteban E, Palanca S, Barragán E, Bolufer P

The majority of BRCA1 and BRCA2 mutation detection procedures include screening methods, all of which are time-consuming. High-resolution melting (HRM) is a promising pre-screening method of gene scanning that combines simplicity and rapid identification of genetic variants. We evaluated HRM in the screening of BRCA1/2 Spanish mutations. We studied 40 BRCA1 and 47 BRCA2 DNA samples with different Spanish mutations. We included a group of 20 unknown DNAs from patients with sporadic breast cancer (BC). The assay was performed with the LightCycler((R)) 480 Instrument (Roche). The HRM discriminates all the BRCA1/2 Spanish mutations studied from wild-type DNA. Besides, 54 out of 87 mutations were clearly differentiated from each other. In sporadic BC 11 polymorphisms and three unclassified variants were found in both genes. HRM is a valuable method for rapid screening of BRCA1/2 Spanish mutations and is capable of differentiating new genetic variants in PCR products.



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Source: http://www.hubmed.org/display.cgi?uids=18528753
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Fwd: Successful Salvage Robotic-Assisted Radical Prostatectomy After External Beam Radiotherapy Failure.

---------- Forwarded message ----------
From: HubMed - cancer <rssfwd@rssfwd.com>
Date: Sat, Jun 7, 2008 at 2:25 AM
Subject: Successful Salvage Robotic-Assisted Radical Prostatectomy After External Beam Radiotherapy Failure.
To: mesothelioma77@gmail.com


[1]Urology. 2008 Jun 3;
Jamal K, Challacombe B, Elhage O, Popert R, Kirby R, Dasgupta P

We describe the first case of salvage robotic-assisted radical prostatectomy for local recurrence after external beam radiotherapy. A 50-year-old man initially underwent combined external beam radiotherapy and hormonal treatment for Stage T2a prostate adenocarcinoma. The prostate-specific antigen level was 10.5 ng/mL, and the Gleason score was 3+3. Two years later, he developed biopsy-proven recurrent disease. He underwent salvage robotic-assisted radical prostatectomy. The patient was discharged on day 1 postoperatively. The histologic analysis revealed an organ-confined tumor. His prostate-specific antigen at 3 months was

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Source: http://www.hubmed.org/display.cgi?uids=18533232
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